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KMID : 0811720060100000341
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Korean Journal of Physiology & Pharmacology
2006 Volume.10 No. 0 p.341 ~ p.0
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BLEBBISTATIN, AN INHIBITOR OF MYOSIN II, SUPPRESSES THE FORCE GENERATION IN BETA-ESCIN SKINNED SMOOTH MUSCLE FROM GUINEA PIG TAENIA CAECI
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Watanabe Masaru
Kohama Kazuhiro
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Abstract
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Blebbistatin, a potent inhibitor of striated muscle myosin II and vertebrate nonmuscle myosin II, is known to poorly inhibit the smooth muscle myosin ATPase activity (Limouze et al., 2004). Recent studies using intact smooth muscle preparations (e.g. Rhee et al., 2006) presented inhibitory effects of blebbistatin on the contraction at least in some experimental conditions, however. We, therefore, studied the effects of blebbistatin on the mechanical responses of beta escin skinned preparations from guinea pig taenia caeci, to evaluate the blebbistatin action on the smooth muscle contractile elements in detail. Blebbistatin inhibited both Ca2+ induced contraction with only little effects on the Ca2+ sensitivity for the force, and 30 mM Mg2+ induced, myosin phosphorylation independent, force development. These effects of blebbistatin were highly temperature dependent. Also, blebbistatin accelerated relaxation by Ca2+ removal. IC50 values of the blebbistatin effects presented above were nearly 25 micro M. The present results suggested that blebbistain directly interfare cross-bridge interaction. The mechanisms of blebbistatin action on the smooth muscle contractile elements will be discussed.
Source: Korean J Physiol Pharmacol.2006 Oct;10(Suppl II):246
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